Naslov (srp)

Ispitivanje interakcije fotoosetljivog nanokompozitnog sistema baziranog na nedopiranim i dopiranim česticama titanijum (IV) oksida s biomolekulima i ćelijama

Autor

Matijević, Milica, 1984-

Doprinosi

Đorđević, Dragan, 1973-
Nakarada, Đura, 1987-
Nešić, Maja
Korićanac, Lela

Opis (srp)

Nanostructured inorganic compounds such as TiO2 may be used as drug delivery carriersand photosensitizers (PSs) in light-based therapy – photodynamic therapy (PDT). Thisdoctoral dissertation explores the implementation of PDT with unmodified TiO2 (colloidalnanoparticles, TiO2 NPs; prolate nanospheroids, TiO2 PNSs) and modified (dopednanoparticles, N- and C-TiO2 NPs; conjugated to a cytostatic model in the nanocompositesystems, NCSs).Transmission Electron Microscopy confirmed the nanosize (5-104 nm), whereas X-raydiffraction and Raman spectroscopy revealed predomination of the most photo-activeform – anatase of the investigated TiO2. Afterwards, NCSs were formed by coordinationof TiO2 NPs and PNS to the Ru(II) complex with cytostatic properties – cisdichlorobis(2,2'-bipyridyl-4,4'-dicarboxylic acid) ruthenium(II). For the modified TiO2,UV-Vis spectroscopy and bandgap calculations confirmed the visible light absorption.Electron paramagnetic resonance spectroscopy revealed the presence of hydroxyl radicalin water suspensions of TiO2 PNSs and C-TiO2 NPs, whereas no reactive oxygen specieswere detected in suspensions of TiO2 NPs and N-TiO2 NPs.For NCS, the long-term constant release profiles of the Ru(II) complex were establishedin vitro. Namely, the complex release at pH 7 induced by visible light was slowercompared to UV and light’s absence. Furthermore, additional encapsulation of NCS in thesmall unilamellar vesicles significantly suppressed the complex release but with no lightinducedeffect.Confocal microscopy revealed good cells’ internalization of the assessed TiO2. Viabilitytests on non-transformed cells upon the incubation with unmodified TiO2 indicated goodbiocompatibility. The photocytotoxic activity of the investigated TiO2 was tested onseveral cancer cell lines. The most significant viability reduction has been achieved by thecombined treatment with C-TiO2 NPs and blue light on the HeLa cell line. Moreover, CTiO2NPs promoted the programmed cells’ death mechanisms, contributing to theirexceptional PS features for PDT.

Opis (srp)

Bibliografija: str. 111-126. Datum odbrane: 30. 05. 2022. General and Inorganic Chemistry

Jezik

srpski

Datum

2022

Licenca

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