Title (srp)

Uticaj pozicije demarkacije preparacije zuba na inflamaciju gingive i promenu sastava oralne mikrobne flore


Jovanović, Marija, 1986-


Kostić, Milena, 1976-
Kesić, Ljiljana
Kocić, Branislava
Milašin, Jelena
Đorđević, Nadica

Description (srp)

The research started from the assumption that the tooth preparation fora Porcelain-Fused-to-Metal (PFM) crowns with the chamfer finish lineposition can cause damage to the surrounding periodontal tissue and achange of the oral microbial flora composition. The aim of the studywas to examine the clinical parameters of inflammation (gingival indexand gingival bleeding index), cytomorphometric changes of thegingiva, biochemical parameters of inflammation (interleukin 1β (IL-1β), vascular endothelial growth factor (VEGF), matrix metalloproteinase9 (MMP-9) and the presence of periodontopathogens (Porphyromonasgingivalis, Aggregatibacter actinomycetemcomitans, Tannerellaforsythia, Prevotella intermedia) in the gingival crevicular fluid, aswell as the saliva microbiological composition before and after toothpreparation and the composition of bioaerosols during the toothpreparation with the chamfer finish line localized subgingivally andequigingivally. The subjects were divided into two groups dependingof the finish line position of the tooth preparation (subgingival andequigingival). The results of the study showed tooth preparation leadsto an increase in the gingival index and gingival bleeding index in bothexperimental groups, with higher values presenting at subgingivalfinish line position. The examined values of the concentration of thebiochemical inflammatory markers IL-1β and VEGF increasedsignificantly immediately after tooth preparation, and graduallydecreased during the observation period, while there was a decrease inthe MMP-9 concentration. The changes of concentration values of thebiochemical parameters depended on the finish line position withgreater statistical significance at the subgingival position of the toothpreparation compared to the equigingival. Tooth preparation led tochanges in all cytomorphometric parameters, which indicate gingivalinflammation, with a greater effect caused by the subgingival finish lineposition. The exposure of periodontopathogens is statistically significantlyhigher in the intervals after tooth preparation, which indicates theinflammatory potential of the prosthodontic procedure, with a higherbacteria presence at the subgingival compared to the equigingival positionduring the entire examination period. As the distance from the workingfield (patients’ oral cavity) increased, the number of bacterial coloniesdecreased proportionally in both examined groups. Qualitatively, the mostrepresented aerobic colonies were Staphylococcus epidermidis, Neisseriaspp, Streptococcus intermedius, while the most represented facultativelyanaerobic bacterial colonies were Haemophilus parainfluanzae andStreptococcus sanguinis in both examined groups. The qualitativemicrobiological composition of the oral cavity is correlated with the presence of colonies originating from bioaerosols. The quantitativerepresentation of aerobic and facultatively anaerobic microorganismsin the oral cavity immediately after tooth preparation was lower in bothexamined groups with an increase in aerobic and a decrease infacultatively anaerobic colonies during the observation period. Thestudy results indicate a reversible gingival tissue inflammation causedby tooth preparation with a greater changes in the subgingival finishline position. The reversibility of the resulting changes indicates a lowpossibility of permanent periodontal tissue damage caused by dailyprosthodontic procedure. Choosing the appropriate clinical procedureprevents the development of periodontal diseases, reduces the possibilityof iatrogenic damage that can threaten the therapeutic effect, andensures the longevity of fixed prosthodontic restorations.

Description (srp)

Biografija autora: list [227-228].Bibliografija: listovi 188-226. Datum odbrane: 07.12.2023 Prosthodontics

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