Title (srp)

Uticaj tri različite hirurške tehnike na histološke i imunohistohemijske karakteristike venskih graftova


Živković, Igor, 1988-


Putnik, Svetozar, 1971-
Milić, Dragan, 1965-
Mićović, Slobodan, 1966-
Krstić, Miljan, 1973-
Velinović, Miloš, 1964-

Description (srp)

Background and Objectives: The saphenous vein is one of the most commonused grafts (SVG) for surgical revascularisation. The mechanism of the SVGsocclusion is still unknown. Surgical preparation techniques have an importantrole in the early and late graft occlusion. Our study analyzed the influence ofthe three different surgical techniques on the histological andimmunohistochemical characteristics of the vein grafts harvested by threedifferent techniques.Methods: Between Jun 2019 and December 2020, 83 patients who underwentsurgical revascularization were prospectively randomly assigned into threegroups according to saphenous vein graft harvesting (conventional (CVH), notouch(NT) and endoscopic (EVH)) technique. The vein graft samples were senton the histological (hematoxylin-eosin staining) and immunohistochemical(CD31, Factor VIII, Caveolin and eNOS) examinations.Results: the CVH, NT, and EVH groups included 27 patients (mean age67.66±5.6), 31 patients (mean age 66.5±7.4) and 25 patients (mean age 66±5.5),respectively. Hematoxylin-eosin staining revealed a lower grade ofmicrostructural vein damage in the NT group (2, IQR 1-2) in comparison withCVH and EVH (3, IQR 2-4), (4, IQR 2-4) respectively (p <0.001).Immunohistochemical examination revealed a high grade of staining in the NTgroup compared to the CVH and EVH group (CD 31 antibody p=0.02,FVIII, p<0.001, Caveolin, p=0.001, and eNOS, p=0.003).The best preservation of the structural vein integrity was in the NT group, whilethe lowest rate of leg wound complication was in the EVH group. Those factsincrease the interest in developing and implementing the endoscopic no-touchtechnique.

Description (srp)

Bibliografija: listovi 76-84. Datum odbrane: 25.09.2023 Surgery

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